Skills

Bioconjugation: Stochastic and site-specific protein conjugation, ADC characterization and purification – FPLC (GE AKTApure 25), Hydrophobic Interaction Chromatography (HIC), Size Exclusion Chromatography (SEC)

Chemistry: Design and execution of multi-step synthesis, methodology development, bioorthogonal chemistry, chemical characterization – NMR, LC/GC-MS, polarimetry, normal/reverse-phase column chromatography

Photophysics: Near-IR dyes, UV-vis/ fluorescence spectroscopy, fluorescence lifetime, absolute quantum yield

Optical Imaging: Confocal imaging, fluorescence correlation spectroscopy, epifluorescence imaging, organelle staining, super-resolution microscopy, IVIS imaging, in vivo SWIR imaging

Analytical techniques: Flow cytometry, HPLC/ LC-MS method development/validation, dynamic light scattering

Biology: In vitro mammalian cell culture, cell viability/ killing assays, small molecule IVIS and SWIR imaging

Scientific Software: PowerBI, R, Python, Spartan, Gaussian, Pymol, ImageJ